Composite

Part:BBa_K4577008

Designed by: Ma Bourgeois   Group: iGEM23_Ionis-Paris   (2023-10-06)


AmoA gene with the tac promotor, the strong bacterial RBS and the lambda t0 terminator

This construct contains the AmoA gene flanked by the tac promoter and the lambda t0 terminator. The main part is the AmoA gene, which encodes for the AMO protein. Its main function is to oxidise NH3 to NH2-OH.

The tac promoter is a strong promoter from E.coli. It consists of trp and lac UV5. [1]

The lambda t0 terminator is derived from phage lambda.[2]

An efficient ribosome binding site RBS is inserted between the promoter and the gene.[3]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 217
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 69


[edit]
Categories
Parameters
None